Wednesday, July 17, 2019

Alcohol Consumption Drosophila Melanogaster

Alcohol white plague in sexu completelyy frust orderd fruit move drosophila melanogaster Ankit Sharma April 8, 2013 BIO 534 Introduction The fruit wing drosophila melanogaster appearance addiction-like behavior towards inebriant where the fly seem to be physiologically dependent on (Atkinson et. al. , 2012). The flies piece of tail thus exhibit a cognitive colony where much(prenominal) behavior is stored in memory and equal behavior is also shown in the future. This addiction is joined to NPY neuropeptide levels which can be found in Drosophila where these neuropeptides serve as feeding stimulants (Shen et. al. , 2005).Alcohol consumption rate is higher(prenominal) when these NPY neuropeptide levels ar suppressed (Wilcox, 2012). Thus, such factors are linked to the idea that fruit flies see food that contains inebriant more than than regular food, and such behavior increases over time (Devineni and Heberlein, 2009). Sexual foiling is also linked to increase in consumpti on of alcohol. Fruit flies consumed less alcohol when they were sexually satisfied because sex raised NPF levels and the flies consumed more alcohol when they were sexually prevent because their NPF levels were suppressed (Azanchi et. l. , 2012). The decide of the experimentation is to determine if sexually foil staminate fruit flies Drosophila melanogaster resort to alcohol after being rejected by females. Thus, the experiment will determine if the account of sexually frustrated fruit flies will be higher where there is alcohol in the environment than non-sexually frustrated fruit flies. The hypothesis to be tested is vigour hypothesis. The unavailing hypothesis states that sexual frustration has no effect on alcohol consumption by male fruit flies.Thus, there will not be a significantly higher number of rejected male fruit flies in an alcohol environment than non-sexually frustrated flies. Materials and Methods Two credit card cylindrical vials were used to place wingless senseless type fruit flies. The vials contained same amount of strong suit for food. Both vials contained regular ordinary for food. The fruit flies were anesthesized and 40 males and 40 females were separated. 20 males were located in a vial with 20 females that were decapitated by a razor blade. 20 separate males were placed in a different vial with 20 females.The experiment was conducted over a period of 4 days. Two more vials were made, both containing 21% alcohol on genius half status of the medium which was the closest percentage available compared to 15% ethyl alcohol used in other experiments. The males with decapitated females were transferred to one of the vials with alcohol. The males with regular females were transferred to the other vial with alcohol. The number of flies kick in on alcohol and normal side of the medium was recorded in both vials for 20 minutes in 1 minute increments. leash trials were conducted.The mean numbers of fruit flies present on the tw o sides of both vials were metrical. The mean was calculated by the summation of the number of fruit flies in each sides of the vials and divided by 120. The null hypothesis was tested by conducting a two-tailed t-test. Results For all the number of fruit flies observed in the two vials (n=120) there was a significant passing in the number of fruit flies in the two vials in the (t=4. 87, df=118, p

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